BAIT

GCD11

SUI4, translation initiation factor eIF2 subunit gamma, L000000677, YER025W

Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID)

GO Process (3)

GO Function (5)

GO Component (4)

Gene Ontology Biological Process

formation of translation preinitiation complex [IDA]

positive regulation of translational fidelity [IMP]

translational initiation [IMP]

Gene Ontology Molecular Function

GTP binding [IMP]
protein binding [IPI]
tRNA binding [IMP]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA]

Gene Ontology Cellular Component

eukaryotic 43S preinitiation complex [IDA]

eukaryotic 48S preinitiation complex [IDA]

eukaryotic translation initiation factor 2 complex [IMP]

multi-eIF complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SUI3

translation initiation factor eIF2 subunit beta, L000002179, YPL237W

Beta subunit of the translation initiation factor eIF2; involved in the identification of the start codon; proposed to be involved in mRNA binding

GO Process (1)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

formation of translation preinitiation complex [IDA, IMP]

Gene Ontology Molecular Function

mRNA binding [IDA]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA, IPI]

Gene Ontology Cellular Component

eukaryotic 43S preinitiation complex [IDA]

eukaryotic translation initiation factor 2 complex [IDA, IMP]

multi-eIF complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The social and structural architecture of the yeast protein interactome.

Michaelis AC, Brunner AD, Zwiebel M, Meier F, Strauss MT, Bludau I, Mann M

Cellular functions are mediated by protein-protein interactions, and mapping the interactome provides fundamental insights into biological systems. Affinity purification coupled to mass spectrometry is an ideal tool for such mapping, but it has been difficult to identify low copy number complexes, membrane complexes and complexes that are disrupted by protein tagging. As a result, our current knowledge of the interactome ... [more]

Nature Nov. 15, 2023; (); [Pubmed: 37968396]

Quantitative Score

2.0 [Score_FDR+correlation]

Throughput

High Throughput

Additional Notes

Protein interactions were identified using statistically significant enrichment of the proteins in the forward and reverse pull-downs, as well as making use of the profile similarities of interacting proteins in a correlation analysis. High confidence interactions have a total score >=2. This score is a sum of the FDR score of the forward pull-down + FDR score of the reverse pull-down + correlation score.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GCD11 SUI3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gavin AC (2006)	High	-	BioGRID	-
GCD11 SUI3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gordiyenko Y (2014)	Low	-	BioGRID	-
GCD11 SUI3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Krogan NJ (2004)	High	-	BioGRID	-
GCD11 SUI3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Alone PV (2008)	Low	-	BioGRID	-
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Dev K (2010)	Low	-	BioGRID	-
GCD11 SUI3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Perzlmaier AF (2013)	Low	-	BioGRID	1174484
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Perzlmaier AF (2013)	Low	-	BioGRID	1174487
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Singh CR (2005)	Low	-	BioGRID	-
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Thompson GM (2000)	Low	-	BioGRID	-
GCD11 SUI3	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Vanselow S (2022)	Low	-	BioGRID	-
SUI3 GCD11	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Vanselow S (2022)	Low	-	BioGRID	-
GCD11 SUI3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Alone PV (2006)	Low	-	BioGRID	-
SUI3 GCD11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Alone PV (2006)	Low	-	BioGRID	-
SUI3 GCD11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Gordiyenko Y (2014)	Low	-	BioGRID	2862968
SUI3 GCD11	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hashimoto NN (2002)	Low	-	BioGRID	-
GCD11 SUI3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hashimoto NN (2002)	Low	-	BioGRID	-
SUI3 GCD11	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Perzlmaier AF (2013)	Low	-	BioGRID	1174495
GCD11 SUI3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Thompson GM (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GCD11

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [IMP]protein binding [IPI]tRNA binding [IMP]translation initiation factor activity [IMP]translation initiation factor binding [IDA]

Gene Ontology Cellular Component

SUI3

Gene Ontology Biological Process

Gene Ontology Molecular Function

mRNA binding [IDA]translation initiation factor activity [IMP]translation initiation factor binding [IDA, IPI]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

The social and structural architecture of the yeast protein interactome.

Quantitative Score

Throughput

Additional Notes

Related interactions

Curated By

GTP binding [IMP]
protein binding [IPI]
tRNA binding [IMP]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA]

mRNA binding [IDA]
translation initiation factor activity [IMP]
translation initiation factor binding [IDA, IPI]