RUVBL2
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA duplex unwinding [IDA, TAS]
- cellular response to UV [IMP]
- cellular response to estradiol stimulus [IMP]
- chromatin organization [TAS]
- chromatin remodeling [IMP]
- establishment of protein localization to chromatin [IMP]
- histone H2A acetylation [IDA]
- histone H4 acetylation [IDA]
- negative regulation of estrogen receptor binding [IMP]
- positive regulation of histone acetylation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein folding [TAS]
- transcriptional activation by promoter-enhancer looping [IMP]
Gene Ontology Molecular Function- ATP-dependent DNA helicase activity [TAS]
- ATPase activity [IDA]
- DNA helicase activity [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- chromatin DNA binding [IDA]
- identical protein binding [IDA, IPI]
- protein binding [IPI]
- unfolded protein binding [TAS]
- ATP-dependent DNA helicase activity [TAS]
- ATPase activity [IDA]
- DNA helicase activity [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- chromatin DNA binding [IDA]
- identical protein binding [IDA, IPI]
- protein binding [IPI]
- unfolded protein binding [TAS]
Gene Ontology Cellular Component
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery.
Co-fractionation/mass spectrometry (CF/MS) enables the mapping of endogenous macromolecular networks on a proteome scale, but current methods are experimentally laborious, resource intensive and afford lesser quantitative accuracy. Here, we present a technically efficient, cost-effective and reproducible multiplex CF/MS (mCF/MS) platform for measuring and comparing, simultaneously, multi-protein assemblies across different experimental samples at a rate that is up to an order ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an EPIC score >=0.625 in applicable cell lines (MCF7, MDA231 or MCF10A)
- MCF10A cell line (score 0.911)
- MCF7 cell line (score 0.801)
- MDA231 cell line (score 0.843)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DYNC1I2 RUVBL2 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | - |
Curated By
- BioGRID