BAIT

CDK1

CDC2, CDC28A, P34CDC2
cyclin-dependent kinase 1
GO Process (46)
GO Function (5)
GO Component (11)

Gene Ontology Biological Process

Homo sapiens
PREY

NPM1

B23, NPM
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
GO Process (23)
GO Function (14)
GO Component (10)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

B23 regulates GADD45a nuclear translocation and contributes to GADD45a-induced cell cycle G2-M arrest.

Gao H, Jin S, Song Y, Fu M, Wang M, Liu Z, Wu M, Zhan Q

Gadd45a is an important player in cell cycle G2-M arrest in response to genotoxic stress. However, the underlying mechanism(s) by which Gadd45a exerts its role in the control of cell cycle progression remains to be further defined. Gadd45a interacts with Cdc2, dissociates the Cdc2-cyclin B1 complex, alters cyclin B1 nuclear localization, and thus inhibits the activity of Cdc2/cyclin B1 kinase. ... [more]

J. Biol. Chem. Mar. 25, 2005; 280(12);10988-96 [Pubmed: 15644315]

Throughput

  • Low Throughput

Curated By

  • BioGRID