Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Ki-1/57 interacts with PRMT1 and is a substrate for arginine methylation.

Passos DO, Bressan GC, Nery FC, Kobarg J

The human 57 kDa Ki-1 antigen (Ki-1/57) is a cytoplasmic and nuclear protein, associated with Ser/Thr protein kinase activity, and phosphorylated at the serine and threonine residues upon cellular activation. We have shown that Ki-1/57 interacts with chromo-helicase DNA-binding domain protein 3 and with the adaptor/signaling protein receptor of activated kinase 1 in the nucleus. Among the identified proteins that ... [more]

FEBS J. Sep. 01, 2006; 273(17);3946-61 [Pubmed: 16879614]

Throughput

  • High Throughput

Curated By

  • BioGRID