BAIT

MAP2K2

CFC4, MAPKK2, MEK2, MKK2, PRKMK2
mitogen-activated protein kinase kinase 2
GO Process (31)
GO Function (7)
GO Component (15)
Homo sapiens
PREY

RHOA

ARH12, ARHA, RHO12, RHOH12
ras homolog family member A
GO Process (29)
GO Function (3)
GO Component (8)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases.

Buljan M, Ciuffa R, van Drogen A, Vichalkovski A, Mehnert M, Rosenberger G, Lee S, Varjosalo M, Pernas LE, Spegg V, Snijder B, Aebersold R, Gstaiger M

Protein kinases are essential for signal transduction and control of most cellular processes, including metabolism, membrane transport, motility, and cell cycle. Despite the critical role of kinases in cells and their strong association with diseases, good coverage of their interactions is available for only a fraction of the 535 human kinases. Here, we present a comprehensive mass-spectrometry-based analysis of a ... [more]

Mol Cell Dec. 06, 2019; 79(3);504-520.e9 [Pubmed: 32707033]

Quantitative Score

  • 102.0 [Protein Abundance Ratio]

Throughput

  • High Throughput

Additional Notes

  • Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a FDR<1% (protein abundance ratio compared to control are reported)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RHOA MAP2K2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
3333629

Curated By

  • BioGRID