An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases.

Buljan M, Ciuffa R, van Drogen A, Vichalkovski A, Mehnert M, Rosenberger G, Lee S, Varjosalo M, Pernas LE, Spegg V, Snijder B, Aebersold R, Gstaiger M

Protein kinases are essential for signal transduction and control of most cellular processes, including metabolism, membrane transport, motility, and cell cycle. Despite the critical role of kinases in cells and their strong association with diseases, good coverage of their interactions is available for only a fraction of the 535 human kinases. Here, we present a comprehensive mass-spectrometry-based analysis of a ... [more]

Mol Cell Dec. 06, 2019; 79(3);504-520.e9 [Pubmed: 32707033]

Quantitative Score

306.0 [Protein Abundance Ratio]

Throughput

High Throughput

Additional Notes

Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a FDR<1% (protein abundance ratio compared to control are reported)

Curated By

BioGRID

Interaction Summary

IKBKE

Gene Ontology Biological Process

Gene Ontology Molecular Function

NF-kappaB-inducing kinase activity [IDA]protein binding [IPI]

Gene Ontology Cellular Component

MTHFD1L

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]formate-tetrahydrofolate ligase activity [IDA]methenyltetrahydrofolate cyclohydrolase activity [IBA]methylenetetrahydrofolate dehydrogenase (NADP+) activity [IBA]protein homodimerization activity [IDA]