BAIT

DBF4

DNA52, LSD7, protein serine/threonine kinase activating protein DBF4, L000000488, S000029149, L000000513, YDR052C
Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

CLB5

B-type cyclin CLB5, L000000353, YPR120C
B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase; CLB5 has a paralog, CLB6, that arose from the whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Cyclin-specific control of ribosomal DNA segregation.

Sullivan M, Holt L, Morgan DO

Following chromosome duplication in S phase of the cell cycle, the sister chromatids are linked by cohesin. At the onset of anaphase, separase cleaves cohesin and thereby initiates sister chromatid separation. Separase activation results from the destruction of its inhibitor, securin, which is triggered by a ubiquitin ligase called the anaphase-promoting complex (APC). Here, we show in budding yeast that ... [more]

Mol. Cell. Biol. Sep. 01, 2008; 28(17);5328-36 [Pubmed: 18591250]

Throughput

  • Low Throughput

Ontology Terms

  • budding (APO:0000024)

Additional Notes

  • double mutant shows increased budding defects

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CLB5 DBF4
Dosage Lethality
Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Low-BioGRID
299987
CLB5 DBF4
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
590490

Curated By

  • BioGRID