An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases.

Buljan M, Ciuffa R, van Drogen A, Vichalkovski A, Mehnert M, Rosenberger G, Lee S, Varjosalo M, Pernas LE, Spegg V, Snijder B, Aebersold R, Gstaiger M

Protein kinases are essential for signal transduction and control of most cellular processes, including metabolism, membrane transport, motility, and cell cycle. Despite the critical role of kinases in cells and their strong association with diseases, good coverage of their interactions is available for only a fraction of the 535 human kinases. Here, we present a comprehensive mass-spectrometry-based analysis of a ... [more]

Mol Cell Dec. 06, 2019; 79(3);504-520.e9 [Pubmed: 32707033]

Quantitative Score

510.0 [Protein Abundance Ratio]

Throughput

High Throughput

Additional Notes

Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a FDR<1% (protein abundance ratio compared to control are reported)

Curated By

BioGRID

Interaction Summary

DYRK1B

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein kinase activity [IDA]transcription coactivator activity [IDA]

CTBP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NAD binding [ISS]RNA polymerase II transcription corepressor activity [IDA]protein C-terminus binding [TAS]protein binding [IPI]protein domain specific binding [IDA, IPI]repressing transcription factor binding [IPI]transcription factor binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS