An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A protein interaction landscape of breast cancer.

Kim M, Park J, Bouhaddou M, Kim K, Rojc A, Modak M, Soucheray M, McGregor MJ, O'Leary P, Wolf D, Stevenson E, Foo TK, Mitchell D, Herrington KA, Munoz DP, Tutuncuoglu B, Chen KH, Zheng F, Kreisberg JF, Diolaiti ME, Gordan JD, Coppe JP, Swaney DL, Xia B, van 't Veer L, Ashworth A, Ideker T, Krogan NJ

Cancers have been associated with a diverse array of genomic alterations. To help mechanistically understand such alterations in breast-invasive carcinoma, we applied affinity purification–mass spectrometry to delineate comprehensive biophysical interaction networks for 40 frequently altered breast cancer (BC) proteins, with and without relevant mutations, across three human breast cell lines. These networks identify cancer-specific protein-protein interactions (PPIs), interconnected and enriched ... [more]

Science Oct. 01, 2021; 374(6563);eabf3066 [Pubmed: 34591612]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

STK11

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]magnesium ion binding [IDA]p53 binding [IDA]protein binding [IPI]protein kinase activator activity [IDA]protein serine/threonine kinase activity [IDA, TAS]

Gene Ontology Cellular Component

IARS2