NSP7
RAB8A
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- GTP catabolic process [IBA, IDA]
- Golgi vesicle fusion to target membrane [IDA]
- Rab protein signal transduction [IBA]
- axonogenesis [ISS]
- cellular response to insulin stimulus [IBA, ISS]
- cilium assembly [IDA, IMP]
- intracellular protein transport [IBA]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- protein localization to plasma membrane [IBA, ISS]
- protein secretion [IBA]
- regulation of exocytosis [IBA]
- synaptic vesicle exocytosis [IBA]
- vesicle docking involved in exocytosis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [TAS]
- centrosome [IDA]
- cilium [IDA]
- cytoplasmic vesicle membrane [TAS]
- extracellular vesicular exosome [IDA]
- nonmotile primary cilium [IDA]
- nucleolus [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
- phagocytic vesicle [IDA]
- plasma membrane [IBA, IDA]
- primary cilium [IDA]
- recycling endosome membrane [IDA]
- secretory granule membrane [IBA]
- synaptic vesicle [IBA]
- trans-Golgi network transport vesicle [IBA]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A comprehensive SARS-CoV-2-human protein-protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets.
Studying viral-host protein-protein interactions can facilitate the discovery of therapies for viral infection. We use high-throughput yeast two-hybrid experiments and mass spectrometry to generate a comprehensive SARS-CoV-2-human protein-protein interactome network consisting of 739 high-confidence binary and co-complex interactions, validating 218 known SARS-CoV-2 host factors and revealing 361 novel ones. Our results show the highest overlap of interaction partners between published ... [more]
Throughput
- High Throughput
Additional Notes
- High confidence binary SARS-CoV-2-human protein interaction
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NSP7 RAB8A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3800313 | |
| NSP7 RAB8A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.8957 | BioGRID | 2652502 | |
| NSP7 RAB8A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.8957 | BioGRID | 2875514 | |
| NSP7 RAB8A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3209638 |
Curated By
- BioGRID