BAIT
PHO85
LDB15, cyclin-dependent serine/threonine-protein kinase PHO85, phoU, L000001431, YPL031C
Cyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle
GO Process (14)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IGI, IMP]
- fungal-type cell wall organization [IGI]
- negative regulation of calcium-mediated signaling [IGI]
- negative regulation of glycogen biosynthetic process [IMP]
- negative regulation of macroautophagy [IMP]
- negative regulation of phosphate metabolic process [IGI]
- negative regulation of sequence-specific DNA binding transcription factor activity [IGI, IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- positive regulation of macroautophagy [IMP]
- protein phosphorylation [IDA]
- regulation of establishment or maintenance of cell polarity [IGI]
- regulation of protein localization [IDA]
- regulation of protein stability [IGI, IMP]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
BMH1
APR6, 14-3-3 family protein BMH1, L000000185, YER177W
14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication
GO Process (12)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- DNA damage checkpoint [IMP]
- Ras protein signal transduction [IGI]
- aggresome assembly [IMP]
- ascospore formation [IGI]
- fungal-type cell wall chitin biosynthetic process [IGI]
- glycogen metabolic process [IGI]
- mitotic spindle orientation checkpoint [IGI]
- negative regulation of apoptotic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [IPI]
- pseudohyphal growth [IGI]
- signal transduction involved in filamentous growth [IGI]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Mapping pathways and phenotypes by systematic gene overexpression.
Many disease states result from gene overexpression, often in a specific genetic context. To explore gene overexpression phenotypes systematically, we assembled an array of 5280 yeast strains, each containing an inducible copy of an S. cerevisiae gene, covering >80% of the genome. Approximately 15% of the overexpressed genes (769) reduced growth rate. This gene set was enriched for cell cycle-regulated ... [more]
Mol. Cell Feb. 03, 2006; 21(3);319-30 [Pubmed: 16455487]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- Overexpression is lethal in a PHO85 deletion background
Curated By
- BioGRID