An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Physical and functional interactome atlas of human receptor tyrosine kinases.

Salokas K, Liu X, Oehman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M

Much cell-to-cell communication is facilitated by cell surface receptor tyrosine kinases (RTKs). These proteins phosphorylate their downstream cytoplasmic substrates in response to stimuli such as growth factors. Despite their central roles, the functions of many RTKs are still poorly understood. To resolve the lack of systematic knowledge, we apply three complementary methods to map the molecular context and substrate profiles ... [more]

EMBO Rep Jun. 07, 2022; 23(6);e54041 [Pubmed: 35384245]

Quantitative Score

0.02 [BFDR Score]

Throughput

High Throughput

Additional Notes

Proximity Label-MS was carried out to identify high confidence protein interactors with a BFDR of less than or equal to 0.05 (BFDR score reported)

Curated By

BioGRID

Interaction Summary

ALK

Gene Ontology Biological Process

Gene Ontology Molecular Function

NF-kappaB-inducing kinase activity [TAS]protein binding [IPI]protein tyrosine kinase activity [IDA]transmembrane receptor protein tyrosine kinase activity [IDA, TAS]

Gene Ontology Cellular Component

ACSL3

Gene Ontology Biological Process

Gene Ontology Molecular Function

long-chain fatty acid-CoA ligase activity [IMP]protein domain specific binding [IPI]protein kinase binding [IPI]