An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The in vivo Interaction Landscape of Histones H3.1 and H3.3.

Siddaway R, Milos S, Coyaud E, Yun HY, Morcos SM, Pajovic S, Campos EI, Raught B, Hawkins C

Chromatin structure, transcription, DNA replication, and repair are regulated via locus-specific incorporation of histone variants and posttranslational modifications that guide effector chromatin-binding proteins. Here we report unbiased, quantitative interactomes for the replication-coupled (H3.1) and replication-independent (H3.3) histone H3 variants based on BioID proximity labeling, which allows interactions in intact, living cells to be detected. Along with a significant proportion of ... [more]

Mol Cell Proteomics Oct. 01, 2022; 21(10);100411 [Pubmed: 36089195]

Throughput

High Throughput

Additional Notes

BioID
FDR less than or equal to 1%

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CBX3 H3F3A	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hauri S (2016)	High	-	BioGRID	-
H3F3A CBX3	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Heo K (2007)	Low	-	BioGRID	-
H3F3A CBX3	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Smallwood A (2012)	Low	-	BioGRID	665524

Curated By

BioGRID

Interaction Summary

H3F3A

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding [IDA]RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]nucleosomal DNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

CBX3

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [IPI]histone methyltransferase binding [IPI]identical protein binding [IPI]protein binding [IPI]protein domain specific binding [IPI]