PGRP-LE
Gene Ontology Biological Process
- defense response [NAS]
- defense response to Gram-negative bacterium [IGI, IMP]
- defense response to Gram-positive bacterium [IDA]
- defense response to bacterium [IMP]
- determination of adult lifespan [IMP]
- immune response [IMP, ISS, TAS]
- peptidoglycan catabolic process [IKR]
- peptidoglycan recognition protein signaling pathway [IDA]
- positive regulation of innate immune response [IMP]
- response to bacterium [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PGRP-LE
Gene Ontology Biological Process
- defense response [NAS]
- defense response to Gram-negative bacterium [IGI, IMP]
- defense response to Gram-positive bacterium [IDA]
- defense response to bacterium [IMP]
- determination of adult lifespan [IMP]
- immune response [IMP, ISS, TAS]
- peptidoglycan catabolic process [IKR]
- peptidoglycan recognition protein signaling pathway [IDA]
- positive regulation of innate immune response [IMP]
- response to bacterium [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A protein interaction map of Drosophila melanogaster.
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PGRP-LE PGRP-LE | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
PGRP-LE PGRP-LE | Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex. | Low | - | FlyBase | - | |
PGRP-LE PGRP-LE | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | FlyBase | - | |
PGRP-LE PGRP-LE | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | FlyBase | - | |
PGRP-LE PGRP-LE | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 3712621 |
Curated By
- BioGRID