BAIT

GAP1

amino acid permease GAP1, L000000667, YKR039W
General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
Saccharomyces cerevisiae (S288c)
PREY

STP1

BAP1, SSY2, L000002132, YDR463W
Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Amino acid signaling in yeast: post-genome duplication divergence of the Stp1 and Stp2 transcription factors.

Wielemans K, Jean C, Vissers S, Andre B

When yeast cells detect external amino acids via their permease-like Ssy1 sensor, the cytosolic precursor forms of Stp1 and Stp2 transcription factors are activated by endoproteolytic removal of their N-terminal domains, a reaction catalyzed by the Ssy5 endoprotease. The processed Stp factors then migrate into the nucleus, where they activate transcription of several amino acid permease genes including AGP1. We ... [more]

J. Biol. Chem. Jan. 08, 2010; 285(2);855-65 [Pubmed: 19906648]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: rna accumulation (APO:0000224)
  • phenotype: inviable (APO:0000112)

Additional Notes

  • GAP1/STP1/STP2 triple mutants are inviable
  • GAP1/STP1/UGA35 triple mutants are inviable
  • genetic complex
  • triple mutants show aberrant gene expression

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GAP1 STP1
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
426376
GAP1 STP1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
426379

Curated By

  • BioGRID