BAIT
BMH1
APR6, 14-3-3 family protein BMH1, L000000185, YER177W
14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, spindle position checkpoint; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; antiapoptotic gene similar to human 14-3-3; BMH1 has a paralog, BMH2, that arose from whole genome duplication
GO Process (12)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- DNA damage checkpoint [IMP]
- Ras protein signal transduction [IGI]
- aggresome assembly [IMP]
- ascospore formation [IGI]
- fungal-type cell wall chitin biosynthetic process [IGI]
- glycogen metabolic process [IGI]
- mitotic spindle orientation checkpoint [IGI]
- negative regulation of apoptotic process [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [IPI]
- pseudohyphal growth [IGI]
- signal transduction involved in filamentous growth [IGI]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
GCN4
AAS101, AAS3, ARG9, amino acid starvation-responsive transcription factor GCN4, L000000683, YEL009C
bZIP transcriptional activator of amino acid biosynthetic genes; activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
GO Process (5)
GO Function (7)
GO Component (1)
Gene Ontology Biological Process
- negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- nitrogen catabolite activation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA, IGI, IMP]
- positive regulation of transcription initiation from RNA polymerase II promoter [IGI, IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IPI]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, IPI]
- RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IMP, IPI]
- TFIID-class binding transcription factor activity [IPI]
- chromatin binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IPI]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA, IMP, IPI]
- RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IMP, IPI]
- TFIID-class binding transcription factor activity [IPI]
- chromatin binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, IMP]
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Deleting the 14-3-3 protein Bmh1 extends life span in Saccharomyces cerevisiae by increasing stress response.
Enhanced stress response has been suggested to promote longevity in many species. Calorie restriction (CR) and conserved nutrient-sensing target of rapamycin (TOR) and protein kinase A (PKA) pathways have also been suggested to extend life span by increasing stress response, which protects cells from age-dependent accumulation of oxidative damages. Here we show that deleting the yeast 14-3-3 protein, Bmh1, extends ... [more]
Genetics Dec. 01, 2009; 183(4);1373-84 [Pubmed: 19805817]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID