KCNN4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RAB1
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IBA, ISA, ISO]
- GTP catabolic process [IBA, ISO]
- Golgi organization [ISO]
- Rab protein signal transduction [IBA]
- autophagic vacuole assembly [IBA, ISO]
- autophagy [ISO]
- cargo loading into COPII-coated vesicle [ISO]
- cell migration [IMP, ISO]
- defense response to bacterium [ISO]
- endocytosis [ISO]
- growth hormone secretion [ISO]
- interleukin-8 secretion [ISO]
- intracellular protein transport [TAS]
- melanosome transport [IMP]
- positive regulation of glycoprotein metabolic process [ISO]
- substrate adhesion-dependent cell spreading [IMP]
- vesicle transport along microtubule [ISO]
- virion assembly [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Anterograde trafficking of KCa3.1 in polarized epithelia is Rab1- and Rab8-dependent and recycling endosome-independent.
The intermediate conductance, Ca2+-activated K+ channel (KCa3.1) targets to the basolateral (BL) membrane in polarized epithelia where it plays a key role in transepithelial ion transport. However, there are no studies defining the anterograde and retrograde trafficking of KCa3.1 in polarized epithelia. Herein, we utilize Biotin Ligase Acceptor Peptide (BLAP)-tagged KCa3.1 to address these trafficking steps in polarized epithelia, using ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID