Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A novel p53 regulator, C16ORF72/TAPR1, buffers against telomerase inhibition.

Benslimane Y, Sanchez-Osuna M, Coulombe-Huntington J, Bertomeu T, Henry D, Huard C, Bonneil E, Thibault P, Tyers M, Harrington L

Telomere erosion in cells with insufficient levels of the telomerase reverse transcriptase (TERT), contributes to age-associated tissue dysfunction and senescence, and p53 plays a crucial role in this response. We undertook a genome-wide CRISPR screen to identify gene deletions that sensitized p53-positive human cells to telomerase inhibition. We uncovered a previously unannotated gene, C16ORF72, which we term Telomere Attrition and ... [more]

Aging Cell Apr. 01, 2021; 20(4);e13331 [Pubmed: 33660365]

Throughput

  • High Throughput

Additional Notes

  • BioID

Curated By

  • BioGRID