BAIT

TUB1

alpha-tubulin TUB1, L000002387, YML085C
Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

BIK1

ARM5, PAC14, L000000178, YCL029C
Microtubule-associated protein; component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Tubulin isotypes optimize distinct spindle positioning mechanisms during yeast mitosis.

Nsamba ET, Bera A, Costanzo M, Boone C, Gupta ML

Microtubules are dynamic cytoskeleton filaments that are essential for a wide range of cellular processes. They are polymerized from tubulin, a heterodimer of ?- and ?-subunits. Most eukaryotic organisms express multiple isotypes of ?- and ?-tubulin, yet their functional relevance in any organism remains largely obscure. The two ?-tubulin isotypes in budding yeast, Tub1 and Tub3, are proposed to be ... [more]

J Cell Biol Dec. 06, 2020; 220(12); [Pubmed: 34739032]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • SGA genetic interaction dataset for the loss of TUB1. All reported hits have P value < 0.05. Negative synthetic genetic integrations have score < -0.08, while positive synthetic integrations have score > 0.08.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BIK1 TUB1
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
-
BIK1 TUB1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
161749
BIK1 TUB1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
BIK1 TUB1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID