BAIT
CAMK2D
CAMKD
calcium/calmodulin-dependent protein kinase II delta
GO Process (32)
GO Function (8)
GO Component (8)
Gene Ontology Biological Process
- cardiac muscle cell contraction [ISS]
- cellular response to calcium ion [TAS]
- cytokine-mediated signaling pathway [TAS]
- endoplasmic reticulum calcium ion homeostasis [ISS]
- interferon-gamma-mediated signaling pathway [TAS]
- negative regulation of sodium ion transmembrane transport [IDA]
- negative regulation of sodium ion transmembrane transporter activity [IDA]
- peptidyl-serine phosphorylation [IDA]
- peptidyl-threonine phosphorylation [IDA]
- positive regulation of cardiac muscle hypertrophy [IMP]
- protein autophosphorylation [IDA]
- protein oligomerization [IDA]
- protein phosphorylation [IDA]
- regulation of cardiac muscle cell action potential [ISS]
- regulation of cardiac muscle cell action potential involved in regulation of contraction [IC]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [IC, TAS]
- regulation of cell communication by electrical coupling [ISS]
- regulation of cell communication by electrical coupling involved in cardiac conduction [IC]
- regulation of cell growth [NAS]
- regulation of cellular localization [IMP]
- regulation of generation of L-type calcium current [ISS]
- regulation of heart contraction [TAS]
- regulation of heart rate by cardiac conduction [IC]
- regulation of histone deacetylase activity [TAS]
- regulation of membrane depolarization [IDA]
- regulation of relaxation of cardiac muscle [IDA]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISS]
- regulation of ryanodine-sensitive calcium-release channel activity [TAS]
- regulation of the force of heart contraction [TAS]
- regulation of transcription from RNA polymerase II promoter [TAS]
- relaxation of cardiac muscle [ISS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NF1
NFNS, VRNF, WSS
neurofibromin 1
GO Process (49)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- MAPK cascade [ISS]
- Ras protein signal transduction [ISS]
- Schwann cell development [ISS]
- actin cytoskeleton organization [ISS]
- adrenal gland development [ISS]
- artery morphogenesis [ISS]
- brain development [ISS]
- camera-type eye morphogenesis [ISS]
- cell communication [ISS]
- cerebral cortex development [ISS]
- cognition [IMP]
- collagen fibril organization [ISS]
- extracellular matrix organization [ISS]
- forebrain astrocyte development [ISS]
- forebrain morphogenesis [ISS]
- heart development [ISS]
- liver development [ISS]
- metanephros development [ISS]
- myelination in peripheral nervous system [ISS]
- negative regulation of MAP kinase activity [ISS]
- negative regulation of MAPK cascade [IMP, ISS]
- negative regulation of Ras protein signal transduction [IBA]
- negative regulation of cell migration [IMP]
- negative regulation of endothelial cell proliferation [IMP]
- negative regulation of fibroblast proliferation [ISS]
- negative regulation of neuroblast proliferation [ISS]
- negative regulation of oligodendrocyte differentiation [ISS]
- negative regulation of protein kinase activity [ISS]
- negative regulation of transcription factor import into nucleus [ISS]
- osteoblast differentiation [ISS]
- peripheral nervous system development [ISS]
- phosphatidylinositol 3-kinase signaling [ISS]
- pigmentation [ISS]
- positive regulation of Ras GTPase activity [IDA, IMP, ISS]
- positive regulation of adenylate cyclase activity [ISS]
- positive regulation of apoptotic process [ISS]
- positive regulation of neuron apoptotic process [ISS]
- regulation of Ras GTPase activity [IMP]
- regulation of angiogenesis [IMP]
- regulation of blood vessel endothelial cell migration [IMP]
- regulation of bone resorption [ISS]
- regulation of cell-matrix adhesion [ISS]
- regulation of glial cell differentiation [ISS]
- response to hypoxia [ISS]
- smooth muscle tissue development [ISS]
- spinal cord development [ISS]
- sympathetic nervous system development [ISS]
- visual learning [ISS]
- wound healing [ISS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity.
Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological cellular functions and drive devastating human diseases. Profiling PPI networks can, therefore, provide critical insight into disease mechanisms and identify new drug targets. Kinases are regulatory nodes in many PPI networks; yet, facile methods to systematically study kinase interactome dynamics are lacking. We describe kinobead competition and correlation analysis (kiCCA), ... [more]
Mol Cell Mar. 02, 2023; 83(5);803-818.e8 [Pubmed: 36736316]
Quantitative Score
- 0.668972345 [kiCCA Pearson R Value]
Throughput
- High Throughput
Additional Notes
- A kinobead competition and correlation analysis (kiCCA) involving a quantitative mass spectrometry-based chemoproteomic method was carried out to identify endogenous kinase interactors.
- High confidence interactions had a kiCCA Pearson R Value >=0.6.
Curated By
- BioGRID