BAIT
CLK4
CDC-like kinase 4
GO Process (1)
GO Function (1)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
GTF2H2
BTF2, BTF2P44, T-BTF2P44, TFIIH, p44
general transcription factor IIH, polypeptide 2, 44kDa
GO Process (20)
GO Function (8)
GO Component (2)
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [TAS]
- ATP catabolic process [IDA]
- DNA repair [TAS]
- G-protein coupled receptor internalization [IMP]
- gene expression [TAS]
- nucleotide-excision repair [TAS]
- nucleotide-excision repair, DNA damage removal [TAS]
- positive regulation of viral transcription [TAS]
- protein phosphorylation [IDA]
- response to UV [TAS]
- termination of RNA polymerase I transcription [TAS]
- transcription elongation from RNA polymerase I promoter [TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase I promoter [TAS]
- transcription from RNA polymerase II promoter [IDA, TAS]
- transcription initiation from RNA polymerase I promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
- translation [TAS]
- viral process [TAS]
Gene Ontology Molecular Function- DNA-dependent ATPase activity [IDA]
- RNA polymerase II carboxy-terminal domain kinase activity [IDA]
- nucleic acid binding [TAS]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [IDA]
- sequence-specific DNA binding transcription factor activity [NAS]
- translation factor activity, nucleic acid binding [TAS]
- DNA-dependent ATPase activity [IDA]
- RNA polymerase II carboxy-terminal domain kinase activity [IDA]
- nucleic acid binding [TAS]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein kinase activity [IDA]
- sequence-specific DNA binding transcription factor activity [NAS]
- translation factor activity, nucleic acid binding [TAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity.
Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological cellular functions and drive devastating human diseases. Profiling PPI networks can, therefore, provide critical insight into disease mechanisms and identify new drug targets. Kinases are regulatory nodes in many PPI networks; yet, facile methods to systematically study kinase interactome dynamics are lacking. We describe kinobead competition and correlation analysis (kiCCA), ... [more]
Mol Cell Mar. 02, 2023; 83(5);803-818.e8 [Pubmed: 36736316]
Quantitative Score
- 0.693428795 [kiCCA Pearson R Value]
Throughput
- High Throughput
Additional Notes
- A kinobead competition and correlation analysis (kiCCA) involving a quantitative mass spectrometry-based chemoproteomic method was carried out to identify endogenous kinase interactors.
- High confidence interactions had a kiCCA Pearson R Value >=0.6.
Curated By
- BioGRID