BAIT
EPHA4
HEK8, SEK, TYRO1
EPH receptor A4
GO Process (13)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- corticospinal tract morphogenesis [ISS]
- fasciculation of motor neuron axon [ISS]
- fasciculation of sensory neuron axon [ISS]
- motor neuron axon guidance [ISS]
- negative regulation of axon regeneration [ISS]
- peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of Rho guanyl-nucleotide exchange factor activity [IDA]
- protein autophosphorylation [IDA]
- regulation of Rac GTPase activity [ISS]
- regulation of Rap GTPase activity [ISS]
- regulation of astrocyte differentiation [ISS]
- regulation of axonogenesis [ISS]
- regulation of dendritic spine morphogenesis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NCK2
GRB4, NCKbeta
NCK adaptor protein 2
GO Process (10)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- T cell activation [NAS]
- axon guidance [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of cell proliferation [TAS]
- positive regulation of T cell proliferation [IMP]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of epidermal growth factor-activated receptor activity [TAS]
- signal complex assembly [NAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity.
Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological cellular functions and drive devastating human diseases. Profiling PPI networks can, therefore, provide critical insight into disease mechanisms and identify new drug targets. Kinases are regulatory nodes in many PPI networks; yet, facile methods to systematically study kinase interactome dynamics are lacking. We describe kinobead competition and correlation analysis (kiCCA), ... [more]
Mol Cell Mar. 02, 2023; 83(5);803-818.e8 [Pubmed: 36736316]
Quantitative Score
- 0.814147106 [kiCCA Pearson R Value]
Throughput
- High Throughput
Additional Notes
- A kinobead competition and correlation analysis (kiCCA) involving a quantitative mass spectrometry-based chemoproteomic method was carried out to identify endogenous kinase interactors.
- High confidence interactions had a kiCCA Pearson R Value >=0.6.
Curated By
- BioGRID