BAIT
PRKAA2
AMPK, AMPK2, AMPKa2, PRKAA
protein kinase, AMP-activated, alpha 2 catalytic subunit
GO Process (22)
GO Function (7)
GO Component (2)
Gene Ontology Biological Process
- carnitine shuttle [TAS]
- cell cycle arrest [TAS]
- cellular lipid metabolic process [TAS]
- cellular response to glucose starvation [ISS]
- cellular response to nutrient levels [ISS]
- energy reserve metabolic process [TAS]
- fatty acid homeostasis [ISS]
- glucose homeostasis [ISS]
- insulin receptor signaling pathway [TAS]
- lipid biosynthetic process [ISS]
- membrane organization [TAS]
- negative regulation of TOR signaling [ISS]
- negative regulation of apoptotic process [ISS]
- positive regulation of autophagy [ISS]
- positive regulation of glycolytic process [ISS]
- protein phosphorylation [TAS]
- regulation of circadian rhythm [ISS]
- regulation of energy homeostasis [ISS]
- regulation of fatty acid biosynthetic process [TAS]
- response to stress [ISS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [TAS]
- nucleoplasm [TAS]
Homo sapiens
PREY
AHCY
SAHH, adoHcyase
adenosylhomocysteinase
GO Process (6)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity.
Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological cellular functions and drive devastating human diseases. Profiling PPI networks can, therefore, provide critical insight into disease mechanisms and identify new drug targets. Kinases are regulatory nodes in many PPI networks; yet, facile methods to systematically study kinase interactome dynamics are lacking. We describe kinobead competition and correlation analysis (kiCCA), ... [more]
Mol Cell Mar. 02, 2023; 83(5);803-818.e8 [Pubmed: 36736316]
Quantitative Score
- 0.704713066 [kiCCA Pearson R Value]
Throughput
- High Throughput
Additional Notes
- A kinobead competition and correlation analysis (kiCCA) involving a quantitative mass spectrometry-based chemoproteomic method was carried out to identify endogenous kinase interactors.
- High confidence interactions had a kiCCA Pearson R Value >=0.6.
Curated By
- BioGRID