BAIT
RPS6KA4
MSK2, RSK-B
ribosomal protein S6 kinase, 90kDa, polypeptide 4
GO Process (14)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- axon guidance [TAS]
- histone H3-S10 phosphorylation [IMP]
- histone H3-S28 phosphorylation [IMP]
- histone phosphorylation [TAS]
- interleukin-1-mediated signaling pathway [IMP]
- intracellular signal transduction [IDA]
- negative regulation of cytokine production [TAS]
- positive regulation of CREB transcription factor activity [TAS]
- positive regulation of NF-kappaB transcription factor activity [TAS]
- positive regulation of histone acetylation [IMP]
- positive regulation of histone phosphorylation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein phosphorylation [IDA]
- regulation of transcription, DNA-templated [IDA]
Gene Ontology Molecular Function
Homo sapiens
PREY
GOPC
CAL, FIG, GOPC1, PIST, dJ94G16.2
golgi-associated PDZ and coiled-coil motif containing
GO Process (5)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity.
Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological cellular functions and drive devastating human diseases. Profiling PPI networks can, therefore, provide critical insight into disease mechanisms and identify new drug targets. Kinases are regulatory nodes in many PPI networks; yet, facile methods to systematically study kinase interactome dynamics are lacking. We describe kinobead competition and correlation analysis (kiCCA), ... [more]
Mol Cell Mar. 02, 2023; 83(5);803-818.e8 [Pubmed: 36736316]
Quantitative Score
- 0.812994364 [kiCCA Pearson R Value]
Throughput
- High Throughput
Additional Notes
- A kinobead competition and correlation analysis (kiCCA) involving a quantitative mass spectrometry-based chemoproteomic method was carried out to identify endogenous kinase interactors.
- High confidence interactions had a kiCCA Pearson R Value >=0.6.
Curated By
- BioGRID