Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity.

Golkowski M, Lius A, Sapre T, Lau HT, Moreno T, Maly DJ, Ong SE

Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological cellular functions and drive devastating human diseases. Profiling PPI networks can, therefore, provide critical insight into disease mechanisms and identify new drug targets. Kinases are regulatory nodes in many PPI networks; yet, facile methods to systematically study kinase interactome dynamics are lacking. We describe kinobead competition and correlation analysis (kiCCA), ... [more]

Mol Cell Mar. 02, 2023; 83(5);803-818.e8 [Pubmed: 36736316]

Quantitative Score

  • 0.686630089 [kiCCA Pearson R Value]

Throughput

  • High Throughput

Additional Notes

  • A kinobead competition and correlation analysis (kiCCA) involving a quantitative mass spectrometry-based chemoproteomic method was carried out to identify endogenous kinase interactors.
  • High confidence interactions had a kiCCA Pearson R Value >=0.6.

Curated By

  • BioGRID