BAIT
STK3
KRS1, MST2
serine/threonine kinase 3
GO Process (13)
GO Function (8)
GO Component (2)
Gene Ontology Biological Process
- apoptotic process [TAS]
- hippo signaling [IDA, TAS]
- intracellular signal transduction [IDA]
- negative regulation of canonical Wnt signaling pathway [IMP]
- positive regulation of apoptotic process [ISS]
- positive regulation of fat cell differentiation [IGI]
- positive regulation of protein binding [IDA]
- positive regulation of protein serine/threonine kinase activity [TAS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IGI]
- protein phosphorylation [IDA]
- protein stabilization [IMP]
- signal transduction [TAS]
- signal transduction by phosphorylation [IBA]
Gene Ontology Molecular Function- ATP binding [IDA]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein dimerization activity [ISS]
- protein kinase activity [TAS]
- protein serine/threonine kinase activator activity [TAS]
- protein serine/threonine kinase activity [EXP, IDA]
- receptor signaling protein serine/threonine kinase activity [IBA]
- ATP binding [IDA]
- magnesium ion binding [IDA]
- protein binding [IPI]
- protein dimerization activity [ISS]
- protein kinase activity [TAS]
- protein serine/threonine kinase activator activity [TAS]
- protein serine/threonine kinase activity [EXP, IDA]
- receptor signaling protein serine/threonine kinase activity [IBA]
Homo sapiens
PREY
MYH6
ASD3, CMD1EE, CMH14, MYHC, MYHCA, SSS3, alpha-MHC
myosin, heavy chain 6, cardiac muscle, alpha
GO Process (17)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- adult heart development [IMP]
- atrial cardiac muscle tissue morphogenesis [IMP]
- cardiac muscle fiber development [ISS]
- in utero embryonic development [ISS]
- muscle contraction [IDA]
- muscle filament sliding [IMP, TAS]
- myofibril assembly [ISS]
- regulation of ATPase activity [ISS]
- regulation of blood pressure [ISS]
- regulation of heart contraction [ISS]
- regulation of heart rate [IDA]
- regulation of the force of heart contraction [IDA, ISS]
- sarcomere organization [ISS]
- striated muscle contraction [IMP, ISS]
- ventricular cardiac muscle tissue morphogenesis [IMP]
- visceral muscle development [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity.
Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological cellular functions and drive devastating human diseases. Profiling PPI networks can, therefore, provide critical insight into disease mechanisms and identify new drug targets. Kinases are regulatory nodes in many PPI networks; yet, facile methods to systematically study kinase interactome dynamics are lacking. We describe kinobead competition and correlation analysis (kiCCA), ... [more]
Mol Cell Mar. 02, 2023; 83(5);803-818.e8 [Pubmed: 36736316]
Quantitative Score
- 0.698853781 [kiCCA Pearson R Value]
Throughput
- High Throughput
Additional Notes
- A kinobead competition and correlation analysis (kiCCA) involving a quantitative mass spectrometry-based chemoproteomic method was carried out to identify endogenous kinase interactors.
- High confidence interactions had a kiCCA Pearson R Value >=0.6.
Curated By
- BioGRID