Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

DDX24 promotes metastasis by regulating RPL5 in non-small cell lung cancer.

Hu X, Li F, Zhou Y, Gan H, Wang T, Li L, Long H, Li B, Pang P

Non-small cell lung cancer (NSCLC) is a leading cause of cancer death, and metastasis is a crucial determinant of increased cancer mortality. DDX24 has garnered increased attention due to its correlation with tumorigenesis and malignant progression. However, the correlation between DDX24 and NSCLC remains unclear.DDX24 expression in NSCLC tissues and survival rate of patients was analyzed using bioinformatic analysis. Transwell ... [more]

Cancer Med Dec. 01, 2022; 11(23);4513-4525 [Pubmed: 35864588]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RPL31 DDX24
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High9.59BioGRID
3004531

Curated By

  • BioGRID