An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

PTP4A2 promotes lysophagy by dephosphorylation of VCP/p97 at Tyr805.

Bai Y, Yu G, Zhou HM, Amarasinghe O, Zhou Y, Zhu P, Li Q, Zhang L, Nguele Meke F, Miao Y, Chapman E, Tao WA, Zhang ZY

Overexpression of PTP4A phosphatases are associated with advanced cancers, but their biological functions are far from fully understood due to limited knowledge about their physiological substrates. VCP is implicated in lysophagy via collaboration with specific cofactors in the ELDR complex. However, how the ELDR complex assembly is regulated has not been determined. Moreover, the functional significance of the penultimate and ... [more]

Autophagy May. 01, 2023; 19(5);1562-1581 [Pubmed: 36300783]

Throughput

High Throughput

Additional Notes

Note: Bait is catalytically inactive substrate-trapping mutant

Curated By

BioGRID

Interaction Summary

PTP4A2

Gene Ontology Biological Process

Gene Ontology Molecular Function

prenylated protein tyrosine phosphatase activity [TAS]

Gene Ontology Cellular Component

HSP90AA1