An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1.

Simonetti B, Daly JL, Simon-Gracia L, Klein K, Weeratunga S, Anton-Plagaro C, Tobi A, Hodgson L, Lewis PA, Heesom KJ, Shoemark DK, Davidson AD, Collins BM, Teesalu T, Yamauchi Y, Cullen PJ

Endosomal sorting maintains cellular homeostasis by recycling transmembrane proteins and associated proteins and lipids (termed "cargoes") from the endosomal network to multiple subcellular destinations, including retrograde traffic to the trans-Golgi network (TGN). Viral and bacterial pathogens subvert retrograde trafficking machinery to facilitate infectivity. Here, we develop a proteomic screen to identify retrograde cargo proteins of the endosomal SNX-BAR sorting complex ... [more]

Proc Natl Acad Sci U S A Jun. 21, 2022; 119(25);e2201980119 [Pubmed: 35696571]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

TGOLN2

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

SIGMAR1

Gene Ontology Biological Process