BAIT

DBF4

DNA52, LSD7, protein serine/threonine kinase activating protein DBF4, L000000488, S000029149, L000000513, YDR052C
Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

TOP2

TOR3, TRF3, DNA topoisomerase 2, L000002320, YNL088W
Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Checkpoint inhibition of origin firing prevents DNA topological stress.

Morafraile EC, Haenni C, Allen G, Zeisner T, Clarke C, Johnson MC, Santos MM, Carroll L, Minchell NE, Baxter J, Banks P, Lydall D, Zegerman P

A universal feature of DNA damage and replication stress in eukaryotes is the activation of a checkpoint-kinase response. In S-phase, the checkpoint inhibits replication initiation, yet the function of this global block to origin firing remains unknown. To establish the physiological roles of this arm of the checkpoint, we analyzed separation of function mutants in the budding yeast Saccharomyces cerevisiae ... [more]

Genes Dev. Dec. 01, 2018; 33(21-22);1539-1554 [Pubmed: 31624083]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: protein/peptide distribution (APO:0000209)

Additional Notes

  • Rad52 foci
  • top2 effect on the sld3/dbf4 double mutant.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TOP2 DBF4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1788BioGRID
1948552

Curated By

  • BioGRID