PREY

PDPK1

PDK1, PDPK2, PDPK2P, PRO0461
3-phosphoinositide dependent protein kinase 1
GO Process (29)
GO Function (6)
GO Component (5)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Activation of PKC?II through nuclear trafficking guided by ?? subunits of trimeric G protein and 14-3-3?.

Min X, Zhang X, Wang S, Kim KM

Conventional members of protein kinase C (PKC) family, including PKC?II, are constitutively phosphorylated on three major motifs and located in the cytosol in a primed state. In response to cellular stimuli, PKC?II is activated through inducible phosphorylation and Mdm2-mediated ubiquitination. In this study, we aimed to identify the activation mechanism of PKC?II, focusing on the signaling cascade that regulate the ... [more]

Life Sci Jan. 01, 2023; 312();121245 [Pubmed: 36503900]

Throughput

  • Low Throughput

Curated By

  • BioGRID