BAIT

PAFAH1B1

LIS1, LIS2, MDCR, MDS, PAFAH
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
Homo sapiens
PREY

GNB2L1

Gnb2-rs1, H12.3, HLC-7, PIG21, RACK1, HLC7
guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
GO Process (24)
GO Function (13)
GO Component (11)
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Pulse-SILAC and interactomics reveal distinct DDB1-CUL4 associated factors (DCAFs), cellular functions, and protein substrates.

Raisch J, Dubois ML, Groleau M, Levesque D, Burger T, Jurkovic CM, Brailly R, Marbach G, McKenna A, Barrette C, Jacques P E, Boisvert FM

Cullin-RING finger ligases (CRLs) represent the largest family of ubiquitin ligases. They are responsible for the ubiquitination of ?20% of cellular proteins degraded through the proteasome, by catalyzing the transfer of E2-loaded ubiquitin to a substrate. Seven Cullins are described in vertebrates. Among them, CUL4 associates with DDB1 to form the CUL4-DDB1 ubiquitin ligase complex, which is involved in protein ... [more]

Mol Cell Proteomics Sep. 07, 2023; ();100644 [Pubmed: 37689310]

Throughput

  • High Throughput

Additional Notes

  • BioID

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GNB2L1 PAFAH1B1
Cross-Linking-MS (XL-MS)
Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

High-BioGRID
3677625

Curated By

  • BioGRID