BAIT

RAD53

LSD1, MEC2, SPK1, serine/threonine/tyrosine protein kinase RAD53, L000001573, YPL153C

DNA damage response protein kinase; required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication; activates the downstream kinase Dun1p; differentially senses mtDNA depletion and mitochondrial ROS; required for regulation of copper genes in response to DNA-damaging agents; relocalizes to cytosol in response to hyoxia

Kinome Project (Sc)

GO Process (8)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

DNA damage checkpoint [IGI]

DNA repair [IMP]

DNA replication initiation [IMP]

deoxyribonucleoside triphosphate biosynthetic process [IMP]

negative regulation of phosphorylation [IMP]

nucleobase-containing compound metabolic process [IGI]

protein localization [IMP]

protein phosphorylation [IDA]

Gene Ontology Molecular Function

DNA replication origin binding [IDA]
protein kinase activity [IDA]
protein serine/threonine/tyrosine kinase activity [IDA]

Gene Ontology Cellular Component

cytosol [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SWI4

ART1, SBF complex DNA-binding subunit SWI4, L000000124, L000002252, YER111C

DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter in response to heat stress [IMP]

positive regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

SBF transcription complex [IDA, IMP, IPI]

nuclear chromatin [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Checkpoint kinase interaction with DNA polymerase alpha regulates replication progression during stress.

Hadjicharalambous A, Whale AJ, Can G, Skehel JM, Houseley JM, Zegerman P

Background: In eukaryotes, replication stress activates a checkpoint response, which facilitates genome duplication by stabilising the replisome. How the checkpoint kinases regulate the replisome remains poorly understood. The aim of this study is to identify new targets of checkpoint kinases within the replisome during replication stress. Methods: Here we use an unbiased biotin proximity-ligation approach in Saccharomyces cerevisiae to identify ... [more]

Wellcome Open Res Sep. 28, 2023; 8();327 [Pubmed: 37766847]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAD53 SWI4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ho Y (2002)	High	-	BioGRID	-
RAD53 SWI4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Smolka MB (2006)	Low	-	BioGRID	-
RAD53 SWI4	Dosage Lethality Dosage Lethality A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.	Sidorova JM (2002)	Low	-	BioGRID	153992

Curated By

BioGRID

Interaction Summary

RAD53

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA replication origin binding [IDA]protein kinase activity [IDA]protein serine/threonine/tyrosine kinase activity [IDA]

Gene Ontology Cellular Component

SWI4

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

Proximity Label-MS

Publication

Checkpoint kinase interaction with DNA polymerase alpha regulates replication progression during stress.

Throughput

Related interactions

Curated By

DNA replication origin binding [IDA]
protein kinase activity [IDA]
protein serine/threonine/tyrosine kinase activity [IDA]

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
sequence-specific DNA binding [IDA]