BAIT

NSP9

ORF1ab, ORF1ab-nsp9, SARS-CoV2 nsp9, SARS-CoV-2 nsp9, R1AB_SARS2, PRO_0000449627, GU280_gp01_nsp9
Non-structural protein 9
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

CDC40

EHB3, PRP17, PRPF17, RP1-71D21.3
cell division cycle 40
Cell Cycle - Mitotic Spindle Assembly Project
Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted antiviral signaling and host translation.

Meyers JM, Ramanathan M, Shanderson RL, Beck A, Donohue L, Ferguson I, Guo MG, Rao DS, Miao W, Reynolds D, Yang X, Zhao Y, Yang YY, Blish C, Wang Y, Khavari PA

Viral proteins localize within subcellular compartments to subvert host machinery and promote pathogenesis. To study SARS-CoV-2 biology, we generated an atlas of 2422 human proteins vicinal to 17 SARS-CoV-2 viral proteins using proximity proteomics. This identified viral proteins at specific intracellular locations, such as association of accessary proteins with intracellular membranes, and projected SARS-CoV-2 impacts on innate immune signaling, ER-Golgi ... [more]

PLoS Pathog Oct. 01, 2021; 17(10);e1009412 [Pubmed: 34597346]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • HEK293T cells
  • High confidence interactions had a SAINT score >= 0.9.

Curated By

  • BioGRID