BAIT

MARK2

EMK-1, EMK1, PAR-1, Par-1b, Par1b
MAP/microtubule affinity-regulating kinase 2
Alzheimer's Disease Project
Autism spectrum disorder (ASD) Project
UPS Project
Homo sapiens
PREY

MARK3

CTAK1, KP78, PAR1A, Par-1a
MAP/microtubule affinity-regulating kinase 3
Alzheimer's Disease Project
UPS Project
GO Process (0)
GO Function (2)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers.

Ito T, Young MJ, Li R, Jain S, Wernitznig A, Krill-Burger JM, Lemke CT, Monducci D, Rodriguez DJ, Chang L, Dutta S, Pal D, Paolella BR, Rothberg MV, Root DE, Johannessen CM, Parida L, Getz G, Vazquez F, Doench JG, Zamanighomi M, Sellers WR

Although single-gene perturbation screens have revealed a number of new targets, vulnerabilities specific to frequently altered drivers have not been uncovered. An important question is whether the compensatory relationship between functionally redundant genes masks potential therapeutic targets in single-gene perturbation studies. To identify digenic dependencies, we developed a CRISPR paralog targeting library to investigate the viability effects of disrupting 3,284 ... [more]

Nat Genet Dec. 01, 2021; 53(12);1664-1672 [Pubmed: 34857952]

Quantitative Score

  • 1.23971e-06 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • CRISPR GI screen
  • Cell Line: PATU8988S_PANCREAS score (1.11121864712335E-05)
  • Cell Line: PK1_PANCREAS score (6.79058529426555E-20)
  • Cell Line: A549_LUNG score (1.23970515274078E-06)
  • Cell Line: GI1_CENTRAL_NERVOUS_SYSTEM score (0.0007583018976419)
  • Cell Line: HS936T_SKIN score (2.32482327572041E-06)
  • Cell Line: HS944T_SKIN score (7.53974193390933E-13)
  • Cell Line: HSC5_SKIN score (1.61755653358252E-06)
  • Cell Line: IPC298_SKIN score (1.56647024995548E-13)
  • Experimental Setup: Timecourse-Synthetic Lethality
  • GIST: A-phenotypic negative genetic interaction
  • Library: Digenic Paralog CRISPR library
  • Significance Threshold: GEMINI FDR < 0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MARK2 MARK3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High9282BioGRID
3484495
MARK2 MARK3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3360013
MARK2 MARK3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High14.57BioGRID
423156

Curated By

  • BioGRID