An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The CRL4DCAF6 E3 ligase ubiquitinates CtBP1/2 to induce apoptotic signalling and promote intervertebral disc degeneration.

Tseng C, Han Y, Lv Z, Song Q, Wang K, Shen H, Chen Z

Inflammation and apoptosis are two important pathological causes of intervertebral disc degeneration (IDD). The crosstalk between these two biological processes during IDD pathogenesis remains elusive. Herein, we discovered that chronic inflammation induced apoptosis through a cullin-RING E3 ligase (CRL)-dependent mechanism. Two cullin proteins, CUL4A and 4B, recruited DNA damage-binding protein 1 (DDB1), RING-box protein 1 (RBX1) and DDB1- and CUL4-associated ... [more]

J Mol Med (Berl) Feb. 01, 2023; 101(1-2);171-181 [Pubmed: 36688959]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DCAF6 USP9X	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Kristensen AR (2012)	High	-	BioGRID	926533

Curated By

BioGRID

Interaction Summary

DCAF6

Gene Ontology Biological Process

Gene Ontology Molecular Function

ligand-dependent nuclear receptor transcription coactivator activity [IDA]

Gene Ontology Cellular Component

USP9X