PDK1
Gene Ontology Biological Process
- cell proliferation [IMP]
- cellular metabolic process [TAS]
- hypoxia-inducible factor-1alpha signaling pathway [IMP]
- intrinsic apoptotic signaling pathway in response to oxidative stress [IMP]
- protein phosphorylation [IDA, IMP]
- pyruvate metabolic process [TAS]
- regulation of acetyl-CoA biosynthetic process from pyruvate [TAS]
- regulation of glucose metabolic process [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRKCB
Gene Ontology Biological Process
- B cell activation [ISS]
- B cell receptor signaling pathway [ISS]
- blood coagulation [TAS]
- histone H3-T6 phosphorylation [IDA]
- lipoprotein transport [TAS]
- negative regulation of glucose transport [ISS]
- negative regulation of insulin receptor signaling pathway [ISS]
- platelet activation [TAS]
- positive regulation of B cell receptor signaling pathway [ISS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISS]
- positive regulation of NF-kappaB transcription factor activity [ISS]
- positive regulation of angiogenesis [ISS]
- positive regulation of vascular endothelial growth factor receptor signaling pathway [ISS]
- protein phosphorylation [TAS]
- regulation of transcription from RNA polymerase II promoter [IMP]
- signal transduction [NAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
PKC?II activation requires nuclear trafficking for phosphorylation and Mdm2-mediated ubiquitination.
PKC?II, a conventional PKC family member, plays critical roles in the regulation of a variety of cellular functions. Here, we employed loss-of-function approaches and mutants of PKC?II with altered phosphorylation and protein interaction behaviors to identify the cellular mechanisms underlying the activation of PKC?II. Our results show that 3-phosphoinositide-dependent protein kinase-1 (PDK1)-mediated constitutive phosphorylation of PKC?II at the activation loop ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRKCB PDK1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID