Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Large-scale phosphomimetic screening identifies phospho-modulated motif-based protein interactions.

Kliche J, Garvanska DH, Simonetti L, Badgujar D, Dobritzsch D, Nilsson J, Davey NE, Ivarsson Y

Phosphorylation is a ubiquitous post-translation modification that regulates protein function by promoting, inhibiting or modulating protein-protein interactions. Hundreds of thousands of phosphosites have been identified but the vast majority have not been functionally characterised and it remains a challenge to decipher phosphorylation events modulating interactions. We generated a phosphomimetic proteomic peptide-phage display library to screen for phosphosites that modulate short linear ... [more]

Mol Syst Biol Jul. 11, 2023; 19(7);e11164 [Pubmed: 37219487]

Throughput

  • High Throughput

Additional Notes

  • Phage display

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TTYH3 YAP1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3269840
YAP1 TTYH3
Protein-peptide
Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

High-BioGRID
-

Curated By

  • BioGRID