PJA2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
BBS2
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [IMP]
- adult behavior [ISS]
- brain morphogenesis [ISS]
- cerebral cortex development [ISS]
- cilium morphogenesis [ISS]
- fat cell differentiation [ISS]
- hippocampus development [ISS]
- melanosome transport [ISS]
- negative regulation of appetite by leptin-mediated signaling pathway [ISS]
- negative regulation of multicellular organism growth [ISS]
- photoreceptor cell maintenance [ISS]
- protein localization [ISS]
- protein localization to organelle [ISS]
- regulation of cilium beat frequency involved in ciliary motility [ISS]
- sperm axoneme assembly [ISS]
- striatum development [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Ubiquitylation of BBSome is required for ciliary assembly and signaling.
Bardet-Biedl syndrome (BBS) is a ciliopathy characterized by retinal degeneration, obesity, renal abnormalities, postaxial polydactyly, and developmental defects. Genes mutated in BBS encode for components and regulators of the BBSome, an octameric complex that controls the trafficking of cargos and receptors within the primary cilium. Although both structure and function of the BBSome have been extensively studied, the impact of ... [more]
Throughput
- Low Throughput
Additional Notes
- The reaction contained E1, UBE2D2 [E2], and PJA2 [E3]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PJA2 BBS2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| BBS2 PJA2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PJA2 BBS2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID