BAIT

NRX-1

CELE_C29A12.4
Protein NRX-1
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Caenorhabditis elegans
PREY

NOL-6

CELE_Y51H7C.11, rpa-6, Y51H7C.11
nol-6 encodes a nucleolar RNA-associated protein (NRAP); in addition to its predicted role in ribosome biogenesis and thus, nucleolar integrity, genetic analysis indicates that nol-6, along with other nucleolar proteins, plays a role in regulation of innate immunity: loss of nol-6 activity via RNAi results in increased resistance to infection by bacterial pathogens in a manner dependent upon cep-1/p53 and sym-1, which encodes a leucine-rich repeat protein, and whose transcription is positively regulated by CEP-1; enhanced resistance of nol-6 mutant animals to P. aeruginosa is also dependent upon DAF-16 and PMK-1.
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Caenorhabditis elegans

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Characterization of the intracellular neurexin interactome by in vivo proximity ligation suggests its involvement in presynaptic actin assembly.

Schaan Profes M, Tiroumalechetty A, Patel N, Lauar SS, Sidoli S, Kurshan PT

Neurexins are highly spliced transmembrane cell adhesion molecules that bind an array of partners via their extracellular domains. However, much less is known about the signaling pathways downstream of neurexin's largely invariant intracellular domain (ICD). Caenorhabditis elegans contains a single neurexin gene that we have previously shown is required for presynaptic assembly and stabilization. To gain insight into the signaling ... [more]

PLoS Biol Jan. 01, 2024; 22(1);e3002466 [Pubmed: 38252619]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • High confidence hits were identified relative to the specific endogenous control strain (neurexin-deltaPBM-TurboID) which differs only in its subcellular neurexin localization pattern (loss of synaptic enrichment).
  • TurboID proximity labeling method to identify protein interactors of neurexin's (NRX-1) intracellular domain.

Curated By

  • BioGRID