BRCA2
Gene Ontology Biological Process
- DNA repair [TAS]
- centrosome duplication [IMP]
- cytokinesis [IDA]
- double-strand break repair [IMP, TAS]
- double-strand break repair via homologous recombination [IDA, TAS]
- histone H3 acetylation [IDA]
- histone H4 acetylation [IDA]
- negative regulation of mammary gland epithelial cell proliferation [IDA]
- nucleotide-excision repair [IMP]
- positive regulation of transcription, DNA-templated [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PARP1
Gene Ontology Biological Process
- DNA repair [TAS]
- cellular response to insulin stimulus [IDA]
- double-strand break repair [IMP]
- gene expression [TAS]
- macrophage differentiation [TAS]
- negative regulation of transcription from RNA polymerase II promoter [TAS]
- protein ADP-ribosylation [IDA]
- protein poly-ADP-ribosylation [IDA]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Genetic Screens Reveal FEN1 and APEX2 as BRCA2 Synthetic Lethal Targets.
BRCA1 or BRCA2 inactivation drives breast and ovarian cancer but also creates vulnerability to poly(ADP-ribose) polymerase (PARP) inhibitors. To search for additional targets whose inhibition is synthetically lethal in BRCA2-deficient backgrounds, we screened two pairs of BRCA2 isogenic cell lines with DNA-repair-focused small hairpin RNA (shRNA) and CRISPR (clustered regularly interspaced short palindromic repeats)-based libraries. We found that BRCA2-deficient cells ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: DLD-1 (BTO:0000391, CELLOSAURUS:CVCL_0248)
- Cell Line: PEO1 (EFO:0005445, CELLOSAURUS:CVCL_2686)
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: DDR targeted library (Elledge, 2019)
- Significance Threshold: EdgeR FDR<0.01
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PARP1 BRCA2 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 2342181 | |
| BRCA2 PARP1 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 2344243 |
Curated By
- BioGRID