BAIT

CKAP5

CHTOG, MSPS, TOG, TOGp, ch-TOG
cytoskeleton associated protein 5
Homo sapiens
PREY

SMC3

BAM, BMH, CDLS3, CSPG6, HCAP, SMC3L1
structural maintenance of chromosomes 3
Homo sapiens

Cross-Linking-MS (XL-MS)

An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

Two-Dimensional Fractionation Method for Proteome-Wide Cross-Linking Mass Spectrometry Analysis.

Jiao F, Yu C, Wheat A, Wang X, Rychnovsky SD, Huang L

Cross-linking mass spectrometry (XL-MS) is an emergent technology for studying protein-protein interactions (PPIs) and elucidating architectures of protein complexes. The development of various MS-cleavable cross-linkers has facilitated the identification of cross-linked peptides, enabling XL-MS studies at the systems level. However, the scope and depth of cellular networks revealed by current XL-MS technologies remain limited. Due to the inherently broad dynamic ... [more]

Anal Chem Mar. 15, 2022; 94(10);4236-4242 [Pubmed: 35235311]

Throughput

  • High Throughput

Additional Notes

  • In vitro cross-linking-mass spectrometry (XL-MS) was carried out using HEK-293 cell lysates and the cross-linking reagent DSSO (disuccinimidyl sulfoxide). High confidence protein interactions were identified based on cross-linked peptides having an FDR =< 0.3%.
  • In vitro cross-linking-mass spectrometry (XL-MS) was carried out using HEK-293 cell lysates and the cross-linking reagent DSSO (disuccinimidyl sulfoxide). High confidence protein interactions were identified based on cross-linked peptides having an FDR =< 0.3%.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SMC3 CKAP5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-

Curated By

  • BioGRID