BAIT

CDC20

PAC5, ubiquitin-protein transferase activating protein CDC20, L000000259, YGL116W
Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

IME2

SME1, protein kinase IME2, L000000862, YJL106W
Serine/threonine protein kinase involved in activation of meiosis; associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p
Kinome Project (Sc)
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Spo13/MEIKIN ensures a Two-Division meiosis by preventing the activation of APC/CAma1 at meiosis I.

Rojas J, Oz T, Jonak K, Lyzak O, Massaad V, Biriuk O, Zachariae W

Genome haploidization at meiosis depends on two consecutive nuclear divisions, which are controlled by an oscillatory system consisting of Cdk1-cyclin B and the APC/C bound to the Cdc20 activator. How the oscillator generates exactly two divisions has been unclear. We have studied this question in yeast where exit from meiosis involves accumulation of the APC/C activator Ama1 at meiosis II. ... [more]

EMBO J Oct. 16, 2023; 42(20);e114288 [Pubmed: 37728253]

Throughput

  • Low Throughput

Ontology Terms

  • protein/peptide accumulation (APO:0000149)

Additional Notes

  • Ime2-C restores Rim4 degradation in PHSL1-CDC20/ama1 mutant cells
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CDC20 IME2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.183BioGRID
1982445

Curated By

  • BioGRID