TWIST1
Gene Ontology Biological Process
- aortic valve morphogenesis [IMP, ISO]
- cardiac neural crest cell development involved in outflow tract morphogenesis [IMP]
- cardiac neural crest cell migration involved in outflow tract morphogenesis [IMP]
- cell differentiation [IMP]
- cell proliferation involved in heart valve development [IMP, ISO]
- cellular response to hypoxia [IMP, ISO]
- cranial suture morphogenesis [IMP]
- embryonic camera-type eye formation [ISO]
- embryonic cranial skeleton morphogenesis [IGI, IMP, ISO]
- embryonic digit morphogenesis [IMP]
- embryonic forelimb morphogenesis [IMP]
- embryonic hindlimb morphogenesis [IMP]
- embryonic limb morphogenesis [IMP]
- embryonic skeletal system morphogenesis [IMP]
- endocardial cushion morphogenesis [IMP]
- eyelid development in camera-type eye [ISO]
- hindlimb morphogenesis [IMP]
- in utero embryonic development [IMP]
- mitral valve morphogenesis [IMP]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [ISO]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of cell differentiation [IDA]
- negative regulation of cellular senescence [ISO]
- negative regulation of double-strand break repair [ISO]
- negative regulation of histone acetylation [IDA]
- negative regulation of histone phosphorylation [ISO]
- negative regulation of molecular function [IDA]
- negative regulation of osteoblast differentiation [IDA, ISO]
- negative regulation of oxidative phosphorylation uncoupler activity [IDA]
- negative regulation of peroxisome proliferator activated receptor signaling pathway [IDA]
- negative regulation of phosphatidylinositol 3-kinase signaling [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of skeletal muscle tissue development [IDA]
- negative regulation of striated muscle tissue development [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of tumor necrosis factor production [IGI]
- neural tube closure [IMP]
- neuron migration [IMP]
- ossification [IMP]
- osteoblast differentiation [IMP]
- positive regulation of cell motility [IMP, ISO]
- positive regulation of endocardial cushion to mesenchymal transition involved in heart valve formation [IDA]
- positive regulation of epithelial cell proliferation [ISO]
- positive regulation of epithelial to mesenchymal transition [IMP, ISO]
- positive regulation of fatty acid beta-oxidation [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of interleukin-6 secretion [ISO]
- positive regulation of monocyte chemotactic protein-1 production [ISO]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO, TAS]
- positive regulation of transcription regulatory region DNA binding [ISO]
- positive regulation of tumor necrosis factor production [ISO]
- regulation of bone mineralization [ISO]
- transcription from RNA polymerase II promoter [ISO, TAS]
Gene Ontology Molecular Function- E-box binding [IDA, ISO, TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [TAS]
- bHLH transcription factor binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI, ISO]
- E-box binding [IDA, ISO, TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [TAS]
- bHLH transcription factor binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISO]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI, ISO]
CHD8
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- ATP-dependent chromatin remodeling [ISO]
- DNA duplex unwinding [ISO]
- brain development [ISO]
- canonical Wnt signaling pathway [ISO]
- digestive tract development [ISO]
- in utero embryonic development [IMP]
- negative regulation of Wnt signaling pathway [ISO]
- negative regulation of apoptotic process [IDA, IMP]
- negative regulation of canonical Wnt signaling pathway [ISO]
- negative regulation of fibroblast apoptotic process [IDA]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription from RNA polymerase III promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation.
Protein interaction is critical molecular regulatory activity underlining cellular functions and precise cell fate choices. Using TWIST1 BioID-proximity-labeling and network propagation analyses, we discovered and characterized a TWIST-chromatin regulatory module (TWIST1-CRM) in the neural crest cells (NCC). Combinatorial perturbation of core members of TWIST1-CRM: TWIST1, CHD7, CHD8, and WHSC1 in cell models and mouse embryos revealed that loss of the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TWIST1 CHD8 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | - | |
| TWIST1 CHD8 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | Low | - | BioGRID | - | |
| TWIST1 CHD8 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID