TUP
Gene Ontology Biological Process
- amnioserosa maintenance [IMP]
- axon guidance [IMP]
- chaeta development [IMP]
- chaeta morphogenesis [IMP]
- dendrite guidance [IMP]
- dorsal closure [IMP]
- germ-band shortening [IMP]
- head involution [NAS]
- heart development [IMP]
- lymph gland development [IMP]
- motor neuron axon guidance [IMP]
- muscle cell fate determination [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- notum cell fate specification [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of gene expression [IMP]
- terminal region determination [IGI]
- torso signaling pathway [IGI]
Gene Ontology Molecular Function- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- protein binding [IPI]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- protein binding [IPI]
CHI
Gene Ontology Biological Process
- axon guidance [IMP]
- blastoderm segmentation [TAS]
- chaeta development [IMP]
- chaeta morphogenesis [IMP]
- compound eye development [IMP]
- dendrite guidance [IMP]
- imaginal disc-derived wing morphogenesis [IMP]
- nervous system development [IMP]
- phagocytosis [IMP]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of eclosion [IMP]
- regulation of gene expression [IMP]
- transcription, DNA-templated [IMP]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation large-scale binary protein interaction network for Drosophila melanogaster.
Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of?>?10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of ... [more]
Throughput
- High Throughput
Additional Notes
- interaction identified by large-scale Y2H screening and validated by pairwise testing
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TUP CHI | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 14.9025 | BioGRID | 2866987 | |
| CHI TUP | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| CHI TUP | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | FlyBase | - | |
| TUP CHI | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID