RFC3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RFC4
Gene Ontology Biological Process
- DNA damage checkpoint [IMP]
- DNA repair [TAS]
- DNA replication [ISS, NAS, TAS]
- DNA replication checkpoint [IMP]
- DNA strand elongation involved in DNA replication [NAS, TAS]
- female meiosis chromosome segregation [IMP]
- lateral inhibition [IMP]
- mitotic chromosome condensation [IMP, NAS]
- mitotic nuclear division [IMP]
- neurogenesis [IMP]
- sister chromatid segregation [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation large-scale binary protein interaction network for Drosophila melanogaster.
Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of?>?10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of ... [more]
Throughput
- High Throughput
Additional Notes
- interaction identified by large-scale Y2H screening and validated by pairwise testing
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
RFC3 RFC4 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
RFC4 RFC3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID