RFC3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RFC4
Gene Ontology Biological Process
- DNA damage checkpoint [IMP]
- DNA repair [TAS]
- DNA replication [ISS, NAS, TAS]
- DNA replication checkpoint [IMP]
- DNA strand elongation involved in DNA replication [NAS, TAS]
- female meiosis chromosome segregation [IMP]
- lateral inhibition [IMP]
- mitotic chromosome condensation [IMP, NAS]
- mitotic nuclear division [IMP]
- neurogenesis [IMP]
- sister chromatid segregation [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A protein complex network of Drosophila melanogaster.
Determining the composition of protein complexes is an essential step toward understanding the cell as an integrated system. Using coaffinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly 5,000 individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a statistical framework designed to define individual protein-protein interactions, led to the generation of a ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
RFC4 RFC3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID