BAIT

CEBPA

C/EBP-alpha, CEBP
CCAAT/enhancer binding protein (C/EBP), alpha
Homo sapiens
PREY

SMC1A

CDLS2, DXS423E, SB1.8, SMC1, SMC1L1, SMC1alpha, SMCB, RP6-29D12.1
structural maintenance of chromosomes 1A
Cell Cycle - Mitotic Spindle Assembly Project
Fanconi Anemia Project
Homo sapiens

Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

PRISMA and BioID disclose a motifs-based interactome of the intrinsically disordered transcription factor C/EBP?.

Ramberger E, Sapozhnikova V, Kowenz-Leutz E, Zimmermann K, Nicot N, Nazarov PV, Perez-Hernandez D, Reimer U, Mertins P, Dittmar G, Leutz A

C/EBP? represents a paradigm intrinsically disordered transcription factor containing short linear motifs and post-translational modifications (PTM). Unraveling C/EBP? protein interaction networks is a prerequisite for understanding the multi-modal functions of C/EBP? in hematopoiesis and leukemia. Here, we combined arrayed peptide matrix screening (PRISMA) with BioID to generate an in vivo validated and isoform specific interaction map of C/EBP?. The myeloid C/EBP? ... [more]

iScience Jun. 25, 2021; 24(6);102686 [Pubmed: 34189442]

Throughput

  • High Throughput

Additional Notes

  • Used arrayed peptide matrix screening (PRISMA) method
  • significance threshold (FDR less than 0.1)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CEBPA SMC1A
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
658025

Curated By

  • BioGRID