BAIT

SEM-5

CELE_C14F5.5, C14F5.5
sem-5 encodes a Src homology (SH) domain 2 and 3-containing protein, orthologous to human GRB2 (OMIM:108355) and Drosophila Drk; sem-5 functions in multiple signaling pathways during development including those regulating sex myoblast migration, muscle membrane extension, vulval induction, fluid balance, viability, and formation of the male tail; SEM-5 acts downstream of the LET-23 epidermal growth factor receptor to negatively regulate RAS-, MAP-, and IP-3-, mediated signal transduction; a sem-5::yfp promoter fusion is expressed in many cells throughout development, including the hypodermis, intestine, neurons, body wall muscles, and vulval precursor cells.
Caenorhabditis elegans
PREY

SIP-1

CELE_F43D9.4, SIP/1, sec-1, F43D9.4
sip-1 encodes a member of the small heat shock family of proteins; loss of sip-1 function via antisense or large-scale RNAi experiments results in embryonic lethality and in a small but reproducible reduction in lifespan in otherwise long-lived animals, respectively, suggesting that sip-1 plays a role in embryonic development as well as regulation of adult lifespan; in addition, expression of sip-1 in E. coli is able to confer thermotolerance on the bacterium; sip-1 mRNA is detected in oocytes and developing embryos, with SIP-1 protein abundant and widely expressed in embryos that have not yet begun morphogenesis; sip-1 expression is regulated by the insulin/IGF-1 signaling (IIS) pathway.
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Protein interaction mapping in C. elegans using proteins involved in vulval development.

Walhout AJ, Sordella R, Lu X, Hartley JL, Temple GF, Brasch MA, Thierry-Mieg N, Vidal M

Protein interaction mapping using large-scale two-hybrid analysis has been proposed as a way to functionally annotate large numbers of uncharacterized proteins predicted by complete genome sequences. This approach was examined in Caenorhabditis elegans, starting with 27 proteins involved in vulval development. The resulting map reveals both known and new potential interactions and provides a functional annotation for approximately 100 uncharacterized ... [more]

Science Jan. 07, 2000; 287(5450);116-22 [Pubmed: 10615043]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SEM-5 SIP-1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High5.6111BioGRID
466531
SEM-5 SIP-1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
SEM-5 SIP-1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-WormBase
-

Curated By

  • BioGRID