BAIT

GLD-1

CELE_T23G11.3, T23G11.3
gld-1 encodes a protein containing a K homology (KH) RNA binding domain; GLD-1 is required for regulation of the mitosis/meiosis decision during germline development (promotion of meiotic entry) in parallel with gld-2, gld-3, and nos-3 and also affects spermatogenesis; GLD-1 physically interacts with the 3'- and 5'UTR of its putative mRNA targets in vitro to negatively regulate their translation; GLD-1 also physically interacts with FOG-2, an F-box protein that promotes spermatogenesis; GLD-1 is expressed in the germ cell cytoplasm at high levels during meiotic prophase; GLD-1 phosphorylation and levels in the distal, mitotic germline are negatively regulated by CYE-1/cyclin E, CDK-2, and the RNA-binding protein FBF-1.
Caenorhabditis elegans
PREY

LAF-1

CELE_Y71H2AM.19
Protein LAF-1
GO Process (4)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Caenorhabditis elegans

Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

Publication

Identification of proteins and miRNAs that specifically bind an mRNA in vivo.

Theil K, Imami K, Rajewsky N

Understanding regulation of an mRNA requires knowledge of its regulators. However, methods for reliable de-novo identification of proteins binding to a particular RNA are scarce and were thus far only successfully applied to abundant noncoding RNAs in cell culture. Here, we present vIPR, an RNA-protein crosslink, RNA pulldown, and shotgun proteomics approach to identify proteins bound to selected mRNAs in ... [more]

Nat Commun Sep. 16, 2019; 10(1);4205 [Pubmed: 31527589]

Throughput

  • High Throughput

Additional Notes

  • RNA pulldowns of exogenous gld-1 were carried out to identify high confidence protein interactors that are greater than 4-fold enriched over controls in all replicates and p < 0.01

Curated By

  • BioGRID